Wet Lab: DNA Barcoding - From Samples to Sequences

In this lesson, students perform the wet lab experiments necessary for DNA barcoding. Beginning with a small tissue sample, students purify the DNA, perform the polymerase chain reaction (PCR) using COI-specific primer pools, and analyze their PCR products by agarose gel electrophoresis. PCR reactions that result in products of the correct size are purified and submitted for DNA sequencing. This DNA sequence data can be used in Lesson Nine, or as part of an independent project.

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