CRISPR

Structures of immune cascade complexes from bacteria. These include CRISPR complexes from different bacteria,with guide RNA, target DNA, and the Cas1, Cas2, Cas3, and Cas9 nucleases. This collection of structures can be used to show how bacteria recognize and destroy viral DNA. This system has become an important tool for genome engineering.

iBiology has two nice videos on CRISPR-Cas9

Jennifer Doudna, one of the women who discovered this process, explains how this works in Genome Engineering with CRISPR-Cas9: Birth of a Breakthrough Technology
CRISPR: A word processor for the genome by Youreka Science has some helpful animations.

All the structures below can be viewed in Molecule World on the iPad or Molecule World for iPhone.

We have a tutorial here for exploring the CRISPR Cas9 system in Molecule World.

Bio-Link presented a webinar on Genome Engineering with CRISPR Cas9 on Sept 30th.

Displaying 1 - 9 of 9

Type		Structure Description	PDB ID	Download Structure
An MW Collection		Collections can be downloaded in one step and viewed in the iPad version of Molecule World. Individual structures can be viewed in either version. Download the entire collection of CRISPR structures by clicking the link in the next column. Download individual structures using the links below. View Details		Document CRISPR_2.mwc (2.37 MB)
MMDB	Image	Cas9 from Streptococcus pyogenes with CRISPR RNA about to cut to double-stranded target DNA. View Details	5F9R	Cas9 + CRISPR RNA + target DNA ready to cut Document mmdb_5F9R.cn3 (669.37 KB)
MMDB	Image	Cas9 from Streptococcus pyogenes Cas9 bound to guide RNA and a single-stranded DNA target. View Details	4OO8	Cas9 + guide RNA + target DNA Document mmdb_4OO8.cn3 (580.2 KB)
MMDB	Image	Cas9 from Streptococcus pyogenes with CRISPR RNA bound to double-stranded target DNA. View Details	4UN3	Cas9 + CRISPR RNA + target DNA Document mmdb_4UN3.cn3 (696.55 KB)
MMDB	Image	A Cascade CRISPR surveillance complex from E. coli View Details	4TVX	Cascade complex + guide RNA Document mmdb_4TVX.cn3 (1.78 MB)
MMDB	Image	Another structure of the Cascade CRISPR surveillance complex from E. coli View Details	4U7U	Cascade complex + guide RNA, another view Document mmdb_4U7U.cn3 (2.77 MB)
MMDB	Image	E. coli CRISPR complex with guide RNA bound to target DNA. View Details	4QYZ	CRISPR complex + RNA + target DNA Document mmdb_4QYZ.cn3 (1.39 MB)
MMDB	Image	Cas1 and Cas2, nucleases from E. coli that chop up and store viral DNA. View Details	4P6I	Cas1 and Cas2 Document mmdb_4P6I.cn3 (593.53 KB)
MMDB	Image	Cas3, a nuclease from Thermobifida fusca that attacks viral DNA. View Details	4QQW	Cas3 nuclease Document mmdb_4QQW.cn3 (399.19 KB)