Using molecular models and sequences to understand new technologies: CRISPR/Cas9 in Molecule World™

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Presented at: 
Sequencing Finishing and Analysis in the Future, Santa Fe NM, Jun. 2016 ​Association for Biomolecular Resource Facilities (ABRF), Ft. Lauderdale, FL Feb. 2016
Abstract: 
Todd M. Smith and Sandra G. Porter, Digital World Biology LLC, Seattle WA. CRISPR/Cas9 is a hot topic. CRISPR/Cas9 is not only revolutionizing the field of gene editing, aspects of this system can be used to teach fundamental concepts in immunology and evolution. In terms of immunology, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and restriction/methylation systems protect bacteria from phage. CRISPR sequences are in essence an adaptive immune system for prokaryotes, storing information from previously encountered invaders. Molecular models provide insights in the following ways. Models illustrate the interaction between CRISPR DNA and viral RNA sequences. They show how Cas9 and related proteins can form complexes to cleave viral RNA. Further, as these systems are widespread in bacteria, models can be used to predict and discover new systems. The excitement (and controversy) around CRISPR/Cas9 makes it a useful example for the classroom. Yet, there is a gap between this cutting edge research and educational use. Basic concepts about immunology, evolution, and inheritance of genetic information can be taught. For example, molecular models and sequence comparisons provide hands on ways for students to explore Cas9-related protein structures from bacteria to learn about differences between homology and convergent evolution. To facilitate classroom use, we have created a collection of CRISPR/Cas9 and related structures and have made this freely available at www.digitalworldbiology.com/structure-collections. This collection has been met with enthusiasm by biotechnology education programs (e.g. Madison College, WI) that incorporate CRISPR/Cas9 technologies in their curriculum.
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